MICROBIOLOGY 101 INTERNET TEXT

CHAPTER XVIII: WATER MICROBIOLOGY

As I get time, I will update the notes. As I write the notes I will link them to items both within the document and to addresses outside on the INTERNET that contain relevant information. I will expect you to view that information. Please note that I put KEY WORDS/TERMS IN BOLD, COLORS AND CAPS. These represent terms, definitions and concepts that I consider important. If you have comments please come to see me or contact me through my E-mail at hurlbert@wsu.edu. I will try to answer any questions within 24 hr (during the week) if possible.

 


Updated: 12/4/96 



 

TABLE OF CONTENTS



 

INTRODUCTION

Water is essential for the well being of all living organisms. Evidence indicates that life originated in the primitive ocean approximately 3.5 billion years ago. The blood in our veins closely approximates the composition of sea water. Some biologists even consider terrestrial life an extension of the ocean that has developed the means of packaging sea-water so that it can navigate on dry land or in fresh water (Discover Oct. 1995 pg. 76). Water has shaped evolution so that biochemistry must be considered from the perspective of its relationship to water. In the early lectures I stressed the concepts of the hydrophilic and hydrophobic nature of biological molecules; emphasizing that they are always described in terms of their relationship to water. These water-loving/hating interactions ultimately determine the shape of biological molecules and in so doing, decide the SPECIFICITY that is a basic principle of living processes. You have seen how the change in a single base pair in DNA that results in a subtle alteration in the shape of a crucial molecule can produce a genetic disease. Such alterations usually reflect minuscule interactions between a molecule's components and water.

All microbes live in an aqueous environment. Without water they either perish or become inactive. The ecology of aquatic environments is complex and our understanding of them are limited. Most aquatic environments teem with life. Microbes have evolved that can live in saturated salt (sodium chloride) solutions at temperatures from below freezing to near boiling; they thrive in waters full of toxic substances like copper, cyanide, lead, silver, gasoline, oil, benzene, and a plethora of other noxious natural and man-made substances. It is a NEAR NATURAL LAW that where water, an energy source and basic nutrients exist, you will find microbes living.

Water is of vital importance to man for several reasons. First, it is required to MAINTAIN LIFE. A person may survive for weeks without food, but only a few days without water. Water is crucial for the growing of our food and the operation of all our industries. Finally, it is a major vehicle of a number of water borne diseases that have shaped history in the past and will certainly continue to do so in the future. For example, the Middle East, where we recently went to war, is mostly a desert with a rapidly growing population of diverse peoples who really don't like one another. The Jordan River, the major source of water for Israel, Jordan and Syria, is, by our standards here in the WATER-RICH Northwest, a trickle that we'd laughingly call a "river". Yet, we are willing to defend one or more of the countries in this region, when their survival depends on this TRICKLE OF WATER that's not much larger than the South Fork of the Palouse that runs through Pullman. Many people consider it likely that the next war in the Middle East may be over WATER and not OIL.

The importance of water in our lives is often overlooked because of its seemingly endless supply. Here in the NW it literally rains down upon us much of the year and if we drive a few miles in any direction we cross humongous rivers spanned by impressive bridges, that they tell us once contained a plethora of fish. Further, a half day's trip takes us to the Pacific Ocean, whose immensity and water content boggles the mind. Finally, 70% of the earth is covered with water, which is a whole lot of WET by anyone's definition. Surrounded by this plentiful supply of moisture it is hard to imagine that we could ever be short of water.

However, as I will demonstrate to you, the addition of a few descriptive terms in front of the word WATER changes the entire picture. These words are: CLEAN, POTABLE, POLLUTED and FRESH. We will be considering water from the microbial perspective of disease. Time unfortunately does not permit the examination of other related areas such as the diversity of microbial forms in aquatic habitats and their influence on the ecosystem.

Because of a need for a stable water supply humans built their communities on or near a water supply. However, this has also led to an unfortunate tendency to dispose of our wastes in the same place where we obtain our drinking water. This presented little problem when the population was small and nomadic, for when the stink of a camp became offensive, we picked up and moved the tribe a few miles up or down stream or over the hill to the next stream. Mother nature then stepped in and rapidly recycled the few wastes left behind. With the coming of AGRICULTURE, PERMANENT SETTLEMENTS and LARGE POPULATIONS, it became impossible to move people to a clean habitat; besides someone usually was already there and they generally took serious umbrage when the new arrivals told them to move on. This probably accounts for the bad attitude of the American Indians towards the settlers. This then sets the scenario: A BURGEONING HUMAN POPULATION UNABLE TO MOVE AWAY FROM ITS WASTE.



 

THE VOCABULARY OF WATER MICROBIOLOGY

POTABLE (CLEAN) WATER = Water that is free of all objectionable material, including pathogens, tastes, odors, color, minerals, toxins, radioactive material, organisms, oils, gases etc. It can contain high concentrations of some minerals (e.g. calcium and magnesium) and gases like carbon dioxide; the key word being OBJECTIONABLE. That is, it doesn't have to be toxic or contain pathogens for it to be non-potable.

FRESH WATER = non-salt or -sea water.

POLLUTION = Anything that makes water NON-POTABLE.

SEWAGE = The combined community waste or all the GARBAGE that we and mother nature dump into the sewers of a community. 


These relationships are described in simple MATHEMATICAL terms:

Since the amount of Wt stays roughly constant over the planet whereas the number of humans is INCREASING exponentially, we have a situation illustrated by the following formula: The above formula can now be modified to take into account potable vs polluted water: So the formula can be written to indicate the amount of potable water/human as: It doesn't take a rocket scientist to recognize that certain RELATIONSHIPS follow:

1. As H increases, Wa tends to DECLINE.

2. As U increases, Wa tends to DECLINE.

3. If U and/or H can be decreased, then Wa INCREASES correspondingly. To put it another way, if you take a 5 min shower as opposed to a 20 minute one, there will be more clean water for everyone.

4. As Wp increases the amount of Wc DECREASES and vice versa. In case you don't understand this let me put it to you in another way. The less POOP we put in the potable water the more POOP-FREE water we have to drink.

5. To increase the amount of Wpw the amount of Wp must decrease.

6. The amount of Wp can only be decreased by NOT ADDING pollution to Wt or by REMOVING pollution from Wp.

It turns out that Wc amounts to less than 1% of the Wt and (surprise) it is DECLINING.



 

SOME HISTORY OF ABOUT LAVATORIES AND SEWAGE SYSTEMS

Our ancestors may have been technologically challenged, but they were not stupid. It was easy to deal with human waste when all you had to do was step a few yards into the woods to answer the call of nature, but as towns and the population grew in size, the woods became too distant for routine visits. People soon realized that a little bit of human excreta goes a long ways, aesthetically speaking. As the odor and the flies increased, they eventually decided that something had to be done about getting rid of the unmentionable stuff. So communities came together to work out what to do with all the...well you know....that was piling up and driving down property values. It might be that TAXES were the result of communities having to pool their resources to build defensive barriers to keep out their neighbors and sewage systems to remove their wastes. There are people today who intimately link taxes and feces.

The first recorded toilets and sewers were built around 3,000 BC in several parts of the world, including the Orkney Islands and India/Pakistan. A system of channels on the Orkney islands drained wastes from toilets in the homes into the sea, whereas cities in India/ Pakistan built open drains 2 ft deep by 7-10 inches wide along major streets through which the wastes were flushed away. Cities in Iran/Iraq had communal toilets in 2,300 BC and the Minoans of Crete had toilets and elaborate sewage systems in their palaces about the same time. Athens in 400 BC piped the wastes from homes to cesspools in the streets.

The Romans, being a social people, favored communal toilets that could seat 10 to 20 people in public lavatories located over flowing water to carry the wastes away. It is reported that they carried out business and politics in these facilities. Perhaps this led to the idea expressed by many that politics is "in the toilet". Toilet paper equivalents were a problem and it's likely that most people used a communal cloth and a pot of water (YUK), reusable sponges attached to a stick (YUK again), moss, hay/straw (probably where the term "HARD A.." originated) and/or grass (OUCH). Real toilet paper appeared in China in 589 AD. It is probably true that EVEN TODAY most people on the planet do without toilet paper, which may explain why so many people are in a bad mode much of the time. Larger Roman cities had extensive sewage systems, some of which still function today. The larger drains in Rome were big enough the drive a horse and cart through. In Pompeii one can see the sewage drains running down the center of the streets. Freiburg Germany (where I spent a sabbatical) has a system of channels with flowing water running through the town (in which the community's children now play); these are now "decorative", but in earlier times they served as the town sewers.
 


Figure 1. Functional European water systems in Germany and Italy. The picture on the left shows a flowing channel that runs through the city of Freiburg, Germany; it is for show & is not officially used as a sewer. Those channels are hazards for drunk drivers, who frequently end up in them. The picture on the right shows a fountain in an Italian community that still supplies water; note the support bars where buckets are placed when being filled. Behind the fountain is a channel/drain running down the center of the street.


 


As the population and towns grew in size, the problem of obtaining fresh, clean water also increased. The Roman world is festooned with aqueduct ruins, some of which still work, at least over short distances. A major consideration of every Roman leader was insuring that the citizens of Rome had a constant clean supply of water. Water was brought to Rome from the mountains via an extensive system of aqueducts that spanned mountains and valleys. There were over 250 miles of water conduits inside Rome proper feeding a system of fountains that supplied citizens with fresh water. These fountains are still scattered throughout Rome today. Slaves, labored constantly repairing and maintaining the water system. In India and Pakistan remains of water pipes of clay sealed with asphalt dating back to 2,700 BC have been found. Copper water pipes were used in Egypt as early as 2,450 BC. For more details on ANCIENT TECHNOLOGY read "Ancient Inventions" by P. James & N. Thorpe.
 


Figure 2. Remains of Roman aqueducts. These are remains of Roman aqueducts. The one on the left, behind that handsome family, is in Yugoslavia, whereas the one on the right is in Italy. At least one Roman aqueduct is still in operation in Spain.


 


Every water supply system is constantly in danger of contamination. Before the Germ Theory of Disease few people recognized, other than from an aesthetic standpoint, the need for a "clean" water supply. You have seen how, by using statistics, John Snowin 1855, connected polluted water with human disease. We realize today that throughout history most humans routinely drank contaminated water and that this situation still applies to the majority of humans on the planet. When the population was small and scattered, the outbreaks of water borne diseases were confined to concentrations of humans such as those in a single city or army. However, as population increased and travel accelerated, the combination of improved disease transmission, crowding, poverty and ignorance resulted in extensive water borne epidemics and in the water being increasingly contaminated with human fecal waste containing pathogens. Microbes have evolved to take advantage of water as a mode of transmission of intestinal disease. Consider the advantage in transmission for an intestinal pathogen to induce massive diarrhea in a host. (Evolution of Virulence, Sci. Am. Apr. 1993 pg., 86; Discover Mag. Oct. 1995, pg. 111)

Following the collapse of the Roman empire vestiges of the Roman water technology continued being used throughout Europe. Many villages and towns piped relatively clean water to central fountains from near by streams or springs, or diverted streams through channels in the towns. Centrally placed wells also supplied water in many cities. Sewage disposal continued to be a severe growth-problem as the size of cities grew. Many people, lacking toilets, used chamber pots which they emptied every AM in the streets or alleys. The stench in London in the summers throughout the middle ages and beyond was reported to cause grown men to faint and horses to collapse in their harnesses. Slowly however, the idea of sanitation began to take hold in the 1700s and accelerated through the 1800s. However, Queen Victoria's husband is reported to have died in 1861 of typhoid indicating that even the Royal family apparently drank fecal polluted water. The idea of supplying water to the inhabitants of cities developed along with iron technology that made the manufacturing of inexpensive pipes possible. Private water companies were formed throughout Europe to supply communities. The idea of filtering polluted water through sand to purify it appeared first for private homes of the wealthy and later for municipalities. With the establishment of the relationship between bacterial pathogens and water polluted with human wastes in the late 1800s, and worldwide communication that spread the stories of epidemics, people begin demanding safe water. To meet these demands it, in turn, became necessary to do something about the prodigious quantities of RAW SEWAGE spewing forth from cities and industries into the local water sources. As a consequence, sewage treatment technology developed in the early 1900s, but it was only applied throughout the US in the last 30 years through the passage and enforcement of federal laws like the Clean Water Act. As a microbiologist it is my responsibility to suggest that SERIOUS CONSIDERATION should be given before removing federal regulations on water pollution and water standards.
 


Figure 3. A public fountain still in use in a Swiss mountain town. 


TRIVIA QUESTION: Who is considered the inventor of the modern flush toilet? ANSWER: Thomas Crapper. People said that "he was flush with success". Consider that if you want your name to roll off the lips of millions of people everyday what you have to do. Don't find a cure for cancer or AIDS, or win a war, but invent a better toilet. Then people will constantly refer to you. Like; "You're full of C......" or "That's a lot of C......" or,..... well you can think of other examples I'm sure. 


THE PROBLEM OF OBTAINING POTABLE WATER.

In spite of a SCIENTIFIC UNDERSTANDING of the need for potable water, obtaining and maintaining an unpolluted water supply remains a major problem, even in the DEVELOPED WORLD. Why should this be the case when surely no one wants to drink polluted water; especially water containing human or animal excrement?

The answer is complex. One problem, discussed above, is that people see so much apparently "clean" water around them that it is INCONCEIVABLE that water is in short supply or that it should cost serious MONEY. Another major problem is the PERSPECTIVE that things like water, air and wilderness areas all are part of the COMMONS to be exploited by the many and the RESPONSIBILITY of no one. Finally, there is the problem of the "Frog in the Frying Pan".

A stable potable water supply for a population can only be achieved through community action involving taxation. Unless people see a "clear and present danger", they are generally resistant to increasing their tax burden. As the demands for water increase, the conflicts over its use and the increased cost of keeping it unpolluted or removing the pollutants will continue to cause stress in our society. It is going to take all of our ingenuity to solve these dilemmas. 

 

THE DILEMMA OF THE WETLANDS: AN ILLUSTRATION OF THE COMPLEXITY OF THE PROBLEM

For example, take the situation of WETLANDS. Even the definition of what constitutes a "wetland" is controversial. Is it LAND that is wet all year long or only PART OF THE YEAR? If the latter, how much of the year does it have to be wet to be defined as a wetland? For this example I will define a WETLAND as: An area that is underwater or swampy all the time or for several (6 to 8) months of most years, but when under water, the water may not always be deep. Ecologists have shown that "wetlands" play an important roll in the ecology of the water cycle. These are listed below:

Wetlands act as huge sponges ABSORBING excess water during periods of heavy precipitation.

By serving as a place where EXCESS WATER from rivers and lakes collects and is absorbed, wetlands DECREASE destructive flooding. Coastal wetlands provide important buffers absorbing the force of waves from fierce storms thereby minimizing beach erosion.

The soaking up of the water recharges underground water sources.

Wetlands, especially coastal ones, serve as the habitat for a large number of species that do not exist elsewhere, such as alligators, numerous fish species, and large numbers of aquatic birds. Saltwater marshes are the zones of the HIGHEST BIOLOGICAL PRODUCTIVITY on earth.

Water fowl, like ducks, rely upon wetlands for breeding, feeding and over-wintering.

Wetlands are the largest. LEAST EXPENSIVE and MOST EFFICIENT water purification systems known. The abundant microbial and plant life in wetlands removes vast quantities of pollutants, producing virtually pollutant-free water.
 


FAQ: "With all these benefits, why are wetlands so controversial?"

ANSWER: "Wetlands are often commercially very valuable.", to whit:


 


Because wetlands are so FERTILE, FLAT & SHALLOW they make prime farm land that are inexpensively drained.

Because of their vast area, often on the SEA SHORE or NEAR CITIES, they offer a ready source of inexpensive, flat land that can be inexpensively filled in (e.g. San Francisco Bay).

Because wetlands are seen as COMMERCIALLY UNPRODUCTIVE because they don't directly contribute to personal or business taxable income, they are generally viewed as being "WASTE LANDS".

Coastal wetland are prime areas for commercial and recreational development; the lure to turn "seemingly unproductive, mosquito-ridden-swamp land" into homes, factories, shopping malls and hotels is powerful as it means jobs, homes and tax money for a region and profits for the developers.

Because current ENVIRONMENTAL LAWS prevent commercial development of many wetlands, even those owned privately, there is IMMENSE PRESSURE to remove these restrictions. Senate bill 1373 (offered Fall of '95), for example, would have allowed the commercial development of huge areas of wetlands across the country, but it did not become law. Currently it is estimated that approximately 50% of the wetland of the US have been lost.

Deciding the balance between keeping and commercializing wetlands is difficult. Destruction of duck habitat, for example, lowers bag limits, shortens the duck hunting seasons and negatively effects the businesses supported by that sport. The increased flooding of lands adjacent to rivers because of the loss of wetlands costs society and individuals money in the form of DAMS & DIKES to control the flooding and DISASTER PAYMENTS for people hurt by the floods, as well as funds to CLEAN UP after floods. All this money comes from the GENERAL TAX FUNDS (that's you and me folks). Polluted water that is purified by passage through wetlands washes directly into rivers, lakes and the ocean once the wetlands are eliminated. The USERS of that polluted water now have to pay for the building of additional WATER-PURIFICATION PLANTS to remove the EXTRA pollutants. The loss of fish from the pollution and destruction of the of the saltwater marsh fish-nurseries is reflected in a DECLINE IN FISH CATCHES with a resultant increase in the PRICE of fish and the LOSS OF FISHING JOBS. It is not easy to determine if the increased taxes and job income that results from the conversion of the wetlands to commercial usage completely covers these losses. It will be most interesting to see the outcome of this struggle. 



 

POLLUTANTS AND WHAT GOES ON IN A SEWAGE TREATMENT PLANT

A thorough knowledge of this next section will make you the HIT of your next social event. An erudite discussion of the design & operation of the various types of sewage treatment plants is sure to impress your friends and make you the center of attention, not to mention insuring that you will be invited to all future social occasions.
 


Did you hear about the witch who parked her broom in a NO PARKING zone and she was toad!


 

PRINCIPLE OF SEWAGE TREATMENT

A sewage treatment plant is nothing more than a LARGE MICROBIAL CULTURE FLASK. When a microbiologist inoculates a flask of bacterial medium with a culture the intent is usually to produce more bacteria. However, it can be VIEWED IN ANOTHER WAY. The purpose could be to "USE UP THE NUTRIENTS IN THE MEDIUM" by METABOLIZING them. During the growth of microbes in a medium, the nutrients are oxidized to produce energy and new microbes. The result of this process converts the nutrients to chemicals like carbon dioxide, nitrate, sulfate, phosphate.

Raw sewage is rich in organic nutrients such as human excrement, and food and industrial wastes. The purpose of a sewage treatment plant is to provide the optimum conditions for SELECTING & UTILIZING MICROBES to convert EASILY BIODEGRADABLE ORGANIC MATTER (EBOM) to mineral forms like carbon dioxide, nitrate, sulfate, and phosphate. This process is also called MINERALIZATION. Since microbes grow and utilize nutrients most efficiently under AEROBIC CONDITIONS, sewage treatment plants are designed to provide excess OXYGEN for the microbes.

Finally, there is always some material that can not be easily degraded by microbes which SETTLES OUT at various stages in the treatment process. This material is called SLUDGE and it must also be disposed of as part of the sewage treatment process. There are TWO major designs of SECONDARY sewage treatment plants (STP). These are called the ACTIVATED SLUDGE and the TRICKLING FILTER systems. Each of them achieve the SAME EFFICIENCY of treatment, but they use different DESIGNS for doing so.

STEPS IN SEWAGE TREATMENT; TRICKLING FILTER (TF).

STEP I: The first two stages in BOTH designs are the SAME. The first stage is the PRELIMINARY TREATMENT. In this treatment the raw sewage coming out of the end of the sewage line runs into a large tank. While in this tank the sewage flow slows down enough so that heavy materials like sand and rock settle out and it passes through bars or screens which collect large floating debris like cloth, sticks. The EFFLUENT (any liquid that flows OUT of one container and INTO another) flows into the PRIMARY SETTLING TANK.

STEP II: The PRIMARY SETTLING TANK is a large tank where the flow of sewage slows down to the point where LARGER PIECES OF ORGANIC MATTER settles out as SLUDGE. These tanks also contain a skimmer that picks up smaller floating material, the majority of which are used condoms. We will deal with the sludge later.
 


Figure 4. Settling Tank in STP. This is an empty settling tank at the Pullman STP. Note the sweeping arm at the bottom of the tank that slowly rotates and sweeps the settled sludge into the center where it is removed.


 


STEP III: The third step in the process involves the AEROBIC metabolism of the EBOM. In the TF design this is achieved by spraying the effluent from the primary settling tank over a 4 to 6 foot pile of large inert blocks of material such as rocks, cement blocks, redwood blocks etc. (Fig. 5). The spraying saturates the effluent sewage with oxygen and air circulates between the blocks. The inert blocks become coated with a layer of microbes that are SELECTED because they GROW WELL in the sewage (Fig. 6). This is another example the evolutionary principle of "SURVIVAL OF THE FITTEST". These microbes absorb the nutrients from the sewage as it trickles over them and oxidizes (metabolizes) the EBOM to minerals. Finally the effluent flows into the SECONDARY SETTLING TANK (Fig. 4). Also 99.9% of the pathogens are killed during this step.
 


Figure 5. Trickling filter tank design. The effluent from the primary settling tank flows into the spraying arms, which rotate slowly over the rocks or other inert material. As the effluent passes through the air as a fine spray it is saturated with oxygen. The EBOM is removed from the sewage effluent as it trickles over the rocks.

Figure 6. Inert blocks of material covered with sewage digesting microbes. The microbes include bacteria, molds, algae, protozoa, as well as insect larvae. The microbes feed off of the EBOM that flows past them. The oxidized products of microbial metabolism flows out with the effluent into the secondary settling tanks along with chunks of slime that break off from time to time (Fig. 7).


 


STEP IV: The tank's design is similar to that of the primary settling tank(Fig4). Large debris, consisting mostly of chunks of microbial growth that flakes off the inert blocks, settles out in this tank as sludge. The effluent next flows into the CHLORINATION BASIN.
 


Figure 7. Secondary settling tank at the Pullman STP. Its design is essentially the same as the primary settling tank (Fig 4). Note the duck. The young lady who runs the laboratory (Fall 1995), who is a graduate of this department, tells me that they have trouble with the mother ducks bringing their babies to the tank where the babies get trapped in the drain pipes. When this happens she takes off her shoes and wades around the effluent rim to gather up the babies. Now I call that dedication to ducks and faith in the efficacy of the treated sewage.


 


STEP V: To the effluent of the secondary settling tank is added sufficient CHLORINE to kill most the common bacterial pathogens that have survived the previous treatments. The treated effluent is then released into the environment, usually a nearby stream, river, lake or the ocean. This treatment however, does not kill (STERILIZE) all of the pathogens present so it is unwise to frolic in the water around the sewage outfall.
 


Figure 8. Chlorine treatment of effluent. The photograph on the left shows the large chlorine tanks in the Pullman STP. The chlorine is pumped as a liquid and metered into the effluent from the secondary settling tank. It then flows through the chlorine basins, shown on the right, while the chlorine acts to kill most of the remaining pathogens. The final effluent runs into the environment, in this case the South Fork of the Palouse. 


STEPS IN SEWAGE TREATMENT; ACTIVATED SLUDGE (AS)

Steps I, II, IV & V are the same as those described for the TF treatment, only step III is different. The principle behind the AS treatment is the ADDITION of a microbial inoculum to the effluent from the primary settling tank composed OF HUNGRY MICROBES that have been SELECTED for their ability to grew vigorously on the sewage nutrients in the raw sewage. This selection is achieved by COLLECTING THE SLUDGE from the secondary settling tank (step IV, Fig. 7) and mixing approximately 10% of it with the incoming effluent from the primary settling tank (Fig. 4). This sludge consists of large gelatinous clumps (FLOCS) of microbes that grow in the aeration tanks. When this is done properly the microbes in the sludge are healthy, but RAVENOUSLY HUNGRY; something like college students after a full day of exciting learning. These famished sludge microbes chow down with unrestrained enthusiasm on the juicy sewage (a sort of microbial beer and pizza meal) to which they are added. The entire mixture is mixed vigorously in large 20 feet deep tanks through which enormous quantities of air are BUBBLED; sort of like a huge fish tank without the fish. These tanks are called AERATION TANKS, for obvious reasons. As a result of this the microbes rapidly convert (oxidize) most of the EBOM to the mineral state and in the process most of the pathogens die.
 


Figure 9. Design of an aeration tank. The air, under pressure is pumped into the tanks and vigorously mixed with the sewage to insure that the material always remains aerobic. Otherwise the stench would cause the neighbors to burn the place down. On the far left, some of the activated sludge that has settled out in the secondary settling tank is mixed with the fresh, incoming, sewage from the primary settling tank. The AS contains hungry microbes that immediately chow-down on all that yummy fresh sewage--makes your mouth water at the thought doesn't it?

Figure 10. Aeration tanks of the Pullman AS-STP. The Pullman STP currently has 6 tanks that it operates during the school year. However, during the summer they shut down half of these for obvious reasons. These tanks are about 15 feet deep. This flexibility is another advantage to the AS system. The building in the background houses the large air pumps and the other pumps for moving the sewage around through the system. Note in the foreground the "lifesaver". If you fell in would you want to be saved--maybe if you had kept your mouth shut .


SLUDGE TREATMENT

Anything that SETTLES OUT and is subsequently collected during the sewage treatment process is called SLUDGE. All of the sludge is collected in one place and treated separately from the rest of the sewage material. Sludge treatment involves an ANAEROBIC PROCESS that converts 50% of the organic sludge mainly into methane gas, and some carbon dioxide. To do this the sludge, a disgusting-looking thick black soup, is pumped into large ENCLOSED tanks. Here it is allowed to FERMENT for several weeks while being gently stirred. During this time anaerobic bacteria convert 50% of the organic matter into methane gas. You can usually spot a sewage plant by the presence of one or more large round tanks with a continuous flame burning beside it; this flame is kept burning by the continuous production of the METHANE gas. More modern sewage plants utilize this methane for useful purposes from running city vehicles to heating buildings.
 


Figure 11. The general design and appearance of an anaerobic sludge digestion tank. The picture on the right shows one of the two Pullman sludge digestion tanks. The sludge from the various parts of the STP are pumped into the thickening tank where it further settles out. The thick concentrate is them pumped into the digestion tanks and fermented for several weeks by anaerobic methane-producing Archaeobacteria during which time 50% of the organic matter in the sludge is converted to methane gas.


 


At this point 50% of the original sludge's organic material remains UNDIGESTED and must be DISPOSED of. Usually this sludge, which comes out a very thick black soup, is pumped into shallow excavations in the ground and allowed to dry. Alternatively, if it is safe, it can be pumped onto crop land and used as fertilizer. The dried material is called DRIED SLUDGE (talk about a lack of imagination!). In Pullman and Moscow this is not a lot of this muck, but in places like L.A., Seattle and New York the amount of daily dried sludge produced is so huge that it threatens to cover the city or at least a nearby county or two. Disposing of this sludge presents a really interesting challenge; you might even say large cities are "SLUDGE CHALLENGED". It can be burned (contributing to air pollution), buried into TOXIC LAND FILLS (contributing to ground water pollution) or dumped into the ocean (contributing to their degradation). If it is safe (no heavy metals, toxic organic chemicals and the pathogens killed) it makes an excellent fertilizer for lawns and farms.
 


Figure 12. Pullman STP dried sludge bed. It is about 2 acres in size and approximately 6 feet deep. Pullman sludge is of excellent quality and is safe to use as a fertilizer on farms. Several farms in the area request that the undigested be sprayed on their fields.

Figure 13. Overview of the Pullman STP from the top of the lab/administration building looking south. The activated sludge aeration tanks are hidden behind the building on the far left which contains the pumps for the plant. Note how green the grass is; why is it so GREEN?

Figure 14. Generalized plan of a secondary sewage treatment plant using either (or both) an ACTIVATED SLUDGE or a TRICKLING FILTER SYSTEM. Note the direction of flow of the various sludges and effluents. If you can draw this plan from memory and describe what each component's purpose is, you qualify for the SEWAGE EXPERT gold star and will surely score well on the final exam.


 

COMPARISON OF THE TWO STPs

A FAQ is "Which of the two STP designs is the best?" and "Why chose one over the other?"

 


ANSWER: Both designs are EQUALLY EFFICIENT at removing about 85 to 95% of the input EBOM and both destroy about 99.9% of the pathogens. Both FINAL EFFLUENTS have the same composition and are rich in mineral nutrients like phosphate, nitrate and sulfate. Their initial and operational costs are however different. Generally, TF plants are less expensive to build and to run if land is not EXPENSIVE as they do not require the pumping of large quantities of air into aeration tanks. Conversely, AS systems are more expensive to build and operate. However, the advantage of the AS system is that it can treat LARGER VOLUMES of sewage in a SMALLER SPACE than can TF systems. AS aeration tanks can be stacked up in buildings whereas TF systems can not. Since most land near cities (where the sewage is!) these days is darn near worth its weigh in gold, AS systems are replacing TF systems. A prime example of that is seen in Moscow where they are installing an AS system to replace the TF system.

Neither of the two secondary treatment plants do the following:

They do NOT KILL ALL THE PATHOGENS, particularly certain viruses and protozoan spores that survive passage through the entire plant.

They do NOT DIGEST MANY MAN-MADE ORGANIC CHEMICALS such as pesticides and herbicides.

They do NOT REMOVE TOXIC METALS like cadmium, mercury, and lead. Rather these chemicals, which usually come from industrial process, end up in the sludge.

It does NOT PRODUCE POTABLE WATER. Any water that comes out of the pipe of a secondary sewage treatment plant must be further treated before it is safe to drink. 



 

PRODUCTION OF POTABLE WATER

Given that the vast majority of fresh water on this planet is polluted, what can be done to remove the pollutants INEXPENSIVELY? The last word in the previous sentence is very important as we are once again talking about that four letter word (in most people's minds) TAXES. As water treatment facilities are community based they are supported by a combination of user fees (your water bill) and taxes. At first it might seem perfectly logical that the whole thing could be paid for by USER FEES, since the more water one uses they more you should pay; like gas for your car. It is not that simple however. The largest users of water are agriculture and industries. They have successfully argued that they should NOT be charged the same rate as plain citizens as they use that water to provide food and jobs for society. The difference then is made up by a combination of HIGHER HOUSEHOLD USER FEES and/or TAXES on everyone. What do you think is the most fair way?

Another component of the problem harks back to the matter of perspective discussed previously. People resent paying high fees for something that seems in abundant supply. Further, they resent limitations on their use of water for lawns and washing their cars. 



 


How many of you would not take a job in a community that wouldn't let you wash your car or have a lawn? 



 


When your water source is polluted it obviously must be purified before you will use it in your homes. However, the purification process costs significant money (e.g. user fees and/or taxes). Also the supply systems required for bringing water to homes and for removing the waste water are expensive and need occasional repairs. Since most of us obtain our water from surface sources (e.g. rivers and lakes) how do we control the pollution of these sources? For example, suppose your community's water comes from a river that runs by a city in another state that city DUMPS RAW SEWAGE into the river thereby SAVING ITS CITIZENS the money it would take to build a STP. This means your community has to pay to remove the pollution added by the people upstream. What do you do about that? Go to war with the other city? Pay the extra price and shut up? Sue the SOBs? Move to the city upstream? Fortunately, this is currently not a problem because of the Federal & state regulations that require communities to clean up their waste waters. However, some people are suggesting that this might change if this control is returned to the States. What do you think? Will Idaho citizens tax themselves to clean up their wastes so Washington citizen won't have to spend money removing the Idaho pollutants? Recently a local politician was quoted as being "unable to see how cattle grazing in the wild could be a pollution problem"; obviously the cattle this person was familiar with were toilet-trained & didn't poop near any streams. 


STEPS IN POTABLE WATER PRODUCTION

STORE the polluted water in reservoirs for several weeks. During storage many of the PATHOGENS DIE, some of the EBOM is METABOLIZED and the heavier particles SETTLE to the bottom.

CHEMICAL FLOCCULATION. In this process (also called coagulation) certain nontoxic metal salts like IRON or ALUMINUM are mixed with the water. Then a chemical is added that forms a HEAVY PRECIPITATE (called a floc) that traps particles, including viruses, bacteria, clay etc. and settles to the bottom as sludge.

The cleared effluent is then FILTERED through beds of GRADED SAND AND ROCK to remove the majority of the remaining particles.

The filtered effluent is treated with chlorine to kill any remaining pathogens. It does not render the water sterile however and some pathogens are resistant to the normal levels of chlorine used.
 


Figure 15. The basics of water purification. Polluted water, generally from a reservoir where it has been stored for several months, is pumped into a large mixing tank and Al or Fe salts are mixed in forming a voluminous precipitate. This precipitate traps most of the larger suspended matter of bacteria, algae, viruses and dirt. The mixture is pumped to a settling tank (Fig. 4). The supernatant is then filtered through layers of graded sand beds. This filtration removes most of the remaining pollutants. Finally the water is chlorinated before being sent out to the users.


 


These water purification facilities can be placed in large building; the sand beds being stacked one on top of the other. However, this treatment does not remove such pollutants as radioactive elements, many organic chemicals and some heavy metals. A common problem around the US today is that the high pollution of many water supplies OVERPOWER the ability of the current purification plants to entirely remove pollutants. Thus in many cities of the US the citizens are told NOT TO DRINK THE TAP WATER, but to drink bottled water. This obviously present a dilemma; while paying taxes to build improved water purification facilities may seem undesirable to many, having to buy bottled water amounts to a "TAX". Alternatively, larger doses of chlorine may be added to correct the deficiencies of the purification plant. When the quality of tap water is suspected or when it contains high amounts of chlorine, many people install home purifying systems. Again, this amounts to a hidden TAX for pure water. In addition, a problem with these home-water-purification units is that home owners are unable to VERIFY that the units are DOING THEIR JOB, whereas the municipal facilities have to follow rigorous purity regulations (State & Federal) that require frequent testing of the water for pollutants by trained laboratory personnel. Some people are concerned that if Federal water quality regulations are relaxed the US water quality will further deteriorate. Does this possibility concern you? 



 

HOME WATER PURIFICATION

In many areas of the US, tap water is not considered SAFE TO DRINK because it is polluted. That is, it is not POTABLE WATER. In these places, usually large cities, the problem is often that the community has not been able, or willing, to build needed water purification facilities because of a lack of tax money. What frequently happens is that as the water supply upon which a community is dependent becomes more polluted while the thirsty population has increased. This increases the demand for potable water, however current water purification facilities are unable to meet the demand and the citizens have decided, through their representatives, that increasing the capacity of the water purification plants is not a high priority. To meet the added demand for water, the rate of THROUGHPUT is increased. That is, the amount of water "purified" is increased by passing the polluted water through the purification system FASTER. When this is done, the purification process becomes inefficient and the water is NOT PURIFIED. Often the plant's operators simply increase the amount of chlorine added to the final effluent in an attempt to kill any pathogens that may have passed through the MODIFIED treatment process. However, since they are working at the "edge" of safety, they recommend that citizens should only drink "PURE BOTTLED WATER".

Because bottled water is INCONVENIENT and EXPENSIVE many people install "HOME PURIFICATION SYSTEMS" to remove pollutants. I'm unable to go into a detailed description of the design of these products as there are too many of them and more are appearing all the time as the problem grows. However, the bulk of these units involve ONE OR BOTH of the following components: a FILTRATION SYSTEM to remove microbes and other particles from the tap water and/or ION EXCHANGE RESINS or ACTIVATED CHARCOAL to remove the soluble dangerous minerals (lead, cadmium, mercury.) and/or organics (pesticides, industrial pollutants). The ion exchange resins are the same things used in water softening tanks. Activated charcoal is a form of carbon that has the characteristic of ABSORBING large quantities of organic matter. The filters filter out particles based on the size of the holes in the filters.
 


FAQ about the Home Purification Units are "Are these units effective? and Are they worth buying?":


 


ARE THEY EFFECTIVE?

Some of them are VERY EFFECTIVE when USED AS DESIGNED. That is, you have to KNOW EXACTLY what you are doing before you decide to purchase one of these units. If for example, you are concerned about DANGEROUS METALS like lead, you must purchase a unit (a) that is designed to remove METAL pollutants and not something else (like viruses) and (b) that will remove these pollutants at the RATE you use the water.

All these units FAIL eventually. The more pollutants in the water the sooner they FAIL; the more water you put through them the sooner they FAIL. This means you must REPLACE THE CRITICAL COMPONENTS, usually some form of expensive cartridge, at intervals. When they fail they can CAUSE a pollution problem. A major question you should ask before purchase is "How do you know when a unit is no longer working?"

The buyer must take the RESPONSIBILITY of deciding the kind of unit needed, where it will be placed etc.. The companies selling these items often do not completely inform the buyer as to their design or the sales persons may not be an expert on this item or understand your needs. As is often the case, advertising may OVER-PROMISE the efficacy of a unit or its long term expense.

They are only effective if properly used. Small children enjoy drinking from the ends of hoses or the faucets of bathtubs etc., so a unit on the kitchen sink is probably only going to protect your children a limited percentage of the time. Also do the neighbors, schools and Malls have similar units purifying the water your children and you drink outside the home?

ARE THEY WORTH BUYING?

Certainly, if the water is dangerously polluted and there is NO OTHER REASONABLE ALTERNATIVE. Remember, however that the more expensive the unit the better it probably is.

Before purchasing determine the LONG TERM COST, including replacement costs of disposable components. Companies often make a LARGER PROFIT on replacement parts, because you are committed to using what you have already purchased.

I recommend that before purchasing one contact the local governmental agency in charge of supplying the community's potable water and find out EXACTLY WHAT THE PROBLEMS are. They'll have data defining the pollutants and their quantities in your tap water. They usually can't recommend a particular brand, but armed with that information you are in a much better position to make the correct choice as to which unit to purchase.

Finally, my PERSONAL PERSPECTIVE is that I do not mind paying taxes for a SAFE AND PLENTIFUL WATER SUPPLY. I am willing to pay a LOT for a GUARANTEE that no matter WHERE I GO in my community (or my country for that matter) the water my family drinks will be safe. Few people have the expertise to assess the chemicals and microbial quality of drinking water, but properly run government agencies, employing trained personnel and quality analytical equipment, have a much better chance of producing pure water than you or I do.

One of the COSTS that is important to me is the  PEACE OF MIND that the water I drink is free of all the nasty things described below (and a lot we're not going to get to). Added to this is the fact that once you have one of these units you have added another responsibility to your already FULL AND BUSY LIFE: you must remember to maintain, replace, clean etc. that new Monkey-On-Your-Back. 



 

WATER BORNE DISEASE (WBD)

It is estimated that about one fifth or 20% of Americans drink unsafe or inadequately treated water. This means that you have a ONE IN FIVE chance of being one of them; these might be considered by many to be good lottery odds. The CDC estimates that 940,000 Americans get sick and 900 of them die each year from microbiologically contaminated water. Considering that it might cost billions of dollars to bring these numbers down, do you think that these are these acceptable numbers?

Now I am going to tell you something that you definitely will not like to hear: IF YOU GET SICK FROM POLLUTED WATER IT WILL MEAN THAT YOU HAVE DRUNK WATER CONTAINING HUMAN OR OTHER ANIMAL EXCREMENT. I obviously can't speak for anyone else, but I would be willing to pay a lot of money to insure that that doesn't happen to me; how about you?

The US FDA has published a Hand Book on water and food borne diseases that is more complete than the material given below. Starting in the Spring of 1997 you will be held on exams for the information in that Hand Book on the diseases covered below, with the FOLLOWING EXCEPTIONS: You don't have to know the diagnostic procedures employed to detect and identify the organism; you don't have to know the recent research etc. that requires you to follow other LINKS on the net (but they might be interesting to examine?). 


OBJECTIVES OF THIS SECTION

To learn the major characteristics of the WBDs covered below.

To learn how these diseases are transmitted.

To learn how we can prevent their transmission. 



 

CHARACTERISTICS OF SOME SELECT WBD

CHOLERA

This disease is caused by the gram negative bacterium Vibro cholerae. It is transmitted by fecal contaminated water and food. The bacterium invades a layer of cells lining the intestine. As it grows in these cells it produces a POTENT PROTEIN TOXIN that is responsible for the disease. This toxin causes the normal flow of liquids from the intestine into the blood stream to REVERSE, producing a massive and explosive DIARRHEA of up to 20 liters per day. This in turn cause nausea, vomiting and abdominal pain. The lose of body fluids, along with the ELECTROLYTE SALTS in the blood causes DEHYDRATION and SHOCK. The blood become so thick that the heart is overworked trying to force it through the body. The combination of these effects often leads to death within 24 to 36 hours after onset of the symptoms. Children, in particular die very quickly from the dehydration because they lose liquids and salts MUCH FASTER THAN ADULTS.

You might wonder why should we concern ourselves with a disease most of you probably have never heard of and certainly don't relate to being a problem in the US. Historically, cholera was an important disease in the US until sanitation measures took hold at the beginning of this century. Many more Settlers migrating West died from cholera and typhoid than ever died from Indian attacks. Water on the wagon trains was obtained from barrels using a common drinking cup. What likely happened was that when a member of the wagon train felt the call of nature they would go off into the grass, answer the call and them, like as not, get a drink. Since REST STOPS were rare, water scarce and ignorance rampant, few people washed their hands after relieving themselves of their bodily wastes. So when they dipped the drinking cup and part of their hand in the COMMON water barrel, anything clinging to their hands would be added to the water. Hence water borne disease spread quickly through many a wagon train.

In 1991 a cholera epidemic appeared in Peru and it has been spreading throughout South America ever since. By 1992 there had been 102 cases recorded in the US, mostly from people traveling in SA. There is a constant flow of immigrants, legal and illegal, entering the US from Central and South Am. and they bring with them all the diseases that are endemic to the areas they leave. It is likely that we have a sufficient pool of CHOLERA CARRIERS in the US to start an epidemic if the water supply should ever fail (earthquake, volcanic eruption etc.). We would be wise to remain vigilant where this disease is concerned.

The bad news is that there is currently NO EFFECTIVE VACCINE for cholera and once the disease hits it is too late for antibiotics to do any good. The good news is that the treatment is CHEAP AND READILY AVAILABLE if applied at the first symptoms.

ORAL REHYDRATION THERAPY (ORT)

Since the cause of death in cholera and many other WBD is dehydration and shock, if a victim can be given enough water and electrolytes to replaced those lost, they will recover spontaneously. This treatment is called ORAL REHYDRATION THERAPY (ORT) and consists of making up the following solution:

1/2 teaspoon salt

1/4 teaspoon potassium or sodium bicarbonate or citrate

1/4 teaspoon of potassium chloride

4 tablespoons of glucose, rice, wheat or potato flower
 


Nurses, for more on ORT see Sci. Am. May 1991 pg 50.


 


A reasonable alternative formulation is Ricelyte or a sports-drink sold as a replacement drink for salts lost on hot days. The world wide application of ORT has greatly decreased the death from cholera. For example, cholera victims had a 50 to 60% chance of dying before ORT, but now 97% survive when given this treatment. In places where ORT has been vigorously applied the fatalities from diarrhea fell by as much as 50% and the cost of treatment for diarrhea declined by 75%. A word of warning here. BABIES die very quickly from dehydration and shock and there is a myth in many societies that if a baby is vomiting it should not be given liquids because it hurts them to vomit. THIS IS A SURE WAY TO KILL THE BABY. With few exceptions, a baby losing liquids by vomiting and diarrhea should be given ORT no matter how messy and dirty and VOMIT-COVERED you get (unless you consider a vomit-free house is more important than a live baby). However, ORT requires that busy mothers continuously monitor the sick child.

The prevention of cholera lies in SANITATION (personal & public) and a CLEAN WATER SUPPLY. If you keep fecal material out of the drinking water and off your food you ARE UNLIKELY TO GET CHOLERA. Travelers to countries with ENDEMIC cholera should not eat any food that has not been COOKED or drink anything but BOTTLED DRINKS or water you have BOILED or chemically purified yourself. Asymptomatic carriers exist and the natural reservoir of Cholera has recently been found to be MARINE LIFE. The cholera bacillus is generally not long lived out of its host except in polluted water. 


SALMONELLOSIS

This disease is caused by members of the genus Salmonella, a gram negative rod of the Enterobacteriaceae family. These bacteria are intestinal inhabitants of a wide range of animals including birds, mammals, reptiles and amphibians. Generally, each animal species has a strain that is more or less specific for them, but many of these strains can cross species lines and cause serious intestinal infections in humans. It induces GASTROENTERITIS; diarrhea, abdominal cramps, fever and vomiting. The disease is transmitted by fecal contaminated food or water. There are over 50,000 cases a year reported in the US, but the actual number is likely in the millions. Have you ever had the "runs" and "vomited" for a couple of days without bothering going to the doctor? If so you may have had a case of Salmonellosis.

Various strains differ in virulence. Generally, they invade cells lining the colon and lower small intestine and penetrate the underlying tissues. Their mechanisms of virulence are not totally understood, but exotoxins and endotoxin play a role. Recently a virulent strain of Salmonella enteritidis has appeared in chickens. This strain is particularly DANGEROUS because it infects the ovaries of the chicken and is introduced INSIDE the egg. With other chicken salmonella strains, the bacteria are ONLY IN THE FECES and a contaminated egg shell can be disinfected with a hot soapy wash or chlorine solution. However, the only way the internalized salmonella can be destroyed is by thoroughly HEATING THE EGG THROUGHOUT; that is, a "soft-boiled" or "sunny-side-up" egg is a gamble with your gut, if not your life (before doing this make sure you have a large supply of TP and are near to a toilet). Between 1985 and 1991 49 people died from this organism and >12,000 cases were recorded.

Most cases of Salmonellosis are self limiting, but babies and the elderly can die from the disease. Antibiotics do little good as many of the strains are resistant to numerous antibiotics due to the plasmids they carry. A carrier state is common. Because of the general good quality of the water supply, Salmonellosis is usually a food borne disease in the US. Therefore, to avoid this disease possibly contaminated food like poultry and beef should be sufficiently heated to kill any Salmonella anywhere within the meat. Products made from uncooked egg (e.g. hollandaise sauce) should be avoided unless heated. 


SHIGELLOSIS

This is another gram negative bacterial intestinal pathogen that is transmitted in both fecal contaminated water and food. However, the reservoir of these bacteria are MAINLY HUMAN. It is a major cause of infant mortality around the world. This organism is able to invade and destroy epithelial cells of the colon. It produces diarrhea, often with bloody stools and abdominal cramps. A powerful protein toxin is produced by the organism that plays a role in its virulence. Treatment involves antibiotics and ORT, but drug resistance is a problem because of resistance plasmids. Prevention includes sanitary procedures and proper preparation and cooking of foods. 

TRAVELER'S DIARRHEA AND OTHER RELATED DISEASES

This disease has an interesting history, for it has a way of allowing people to express their PREJUDICES towards other societies by naming the symptoms after a favorite whipping -boy. For example, the English call it Delhi Belly and the Americans Montzuma's Revenge. I suspect the French probably call it something like the London Trots, while WSU students call it the Palouse Lightening. The etiological agent of this disease is Escherichia coli. E. coli exists in a number of strains with EACH GEOGRAPHICAL LOCAL having its own strain. One of these strains, the ENTEROTOXIGENIC strain, induces a gastroenteritis varying from mild to severe in adults. However, it is a frequent cause of death of babies due to the severe diarrhea.

There are three other strains of E. coli that produce diarrhea, but we will only be concerned with one of them, 0157:H7 which will be described in the section on food borne diseases.

Click here for a detailed discussion on Traveler's diarrhea:
 


Figure 16. Electron micrograph of E. coli. Note the dividing cells and the "hairy nature of the cells. This latter is probably the LPS and maybe some capsular material or pili. 



 

HELICOBACTER PYLORI

This bacterium was discovered only a few years ago (1982) by an Australian physician, Dr. B. Marshall. Dr. Marshall observed a SPIRAL SHAPED bacterium in almost all the stomach ULCERS of patients. He began to suspect that they might have something to do with the disease. At first his suggested was considered outrageous as no one thought that bacteria could survive in the acid-filled stomach. Then they suggested that they were a common contaminant that happened to grow in the ulcers after they developed. To settle the argument Dr. Marshall did a very brave, but foolish thing, he DRANK A CULTURE of the organism and promptly became ill. Apparently he didn't develop an ulcer, but others soon repeated his findings and it was then shown that when the bacteria were destroyed by antibiotic treatment, the ulcers cleared up in almost every case. The rest as the say "IS HISTORY".

It is now recognized that 95% of the peptic ulcers and 100% of chronic gastritis is caused by H. pylori. It is also thought that this organism is the major cause of stomach cancer because it produces a persistent immune response that damages the stomach lining. It turns out that it grows in the mucus lining that protects the stomach cells from the strong acid. H. pylori is highly motile and propels itself through the thick mucus by its vigorous corkscrew-motility. Further, they are protected from the stomach's acid by the production of the enzyme urease that breaks down urea into ammonia which NEUTRALIZES the stomach acid in the local of H. pylori.

Its transmission may be via water & person to person, but this has not been rigorously proven yet. However, at least 20% of the people in the US are infected and perhaps many more. It is world wide and is found in a number of animals. Treatment is with antibiotics and bismuth compounds and several groups are working on a vaccine. There is still much to be learned about this organism and how it produces disease, however if it is a major cause of stomach cancer we may see a significant decline in this terrible disease (Sci. Am. Feb. 1996). 


HEPATITIS A

Hepatitis A is a viral WBD disease that can be transmitted by the fecal-oral route (e.g. fecal contaminated water or food) and by sexual intimacy. It produces fatigue, jaundice, fever and diarrhea. It has a long incubation period before the symptoms develop and it is infectious during much of this period. It is treated with gamma globulin (GG; a portion of the blood sera rich in antibodies from a pool of donors) and now with a VACCINE.

The problem is water contaminated with human wastes and food handlers that spread the virus while preparing food during the INCUBATION PERIOD before they become ill. Being a virus, there is no treatment except for the GG. A typical SCENARIO is an announcement in the paper and over the TV that "Everyone who ate at ________ during the period from ___ to ___ should go to their doctor and receive a GG shot". It is a common disease and anywhere from 20 to 80% of a given population will have Ab against this virus. Apparently many people have a mild version in childhood. It is occasionally lethal.


GIARDIA INTESTINALIS

The PROTOZOAN PATHOGEN, Giardia lamblia (or intestinalis) is endemic the US, and is the MOST COMMON identified water-borne illness in the US. It tends to infect individuals who spend a lot of time in the woods, but it also can be contracted from improperly treated city water and from person to person. It is a world-wide pathogen with an incidence often exceeding 10% in underdeveloped countries. The organism infects the small intestine and feeds on the bowel wall. It forms a RESTING CYST that resists adverse conditions. Symptoms vary but often include inflammation of the bowel, diarrhea, cramps, fatigue, vomiting and weight loss. Long term carriers are common. Recovery, followed by repeated attacks may occur.

The reservoirs include beavers, dogs, cats, raccoons and humans. Prevention includes boiling the water or treating it with chlorine or iodine; but it may take several hours in cold water to kill the cyst. It can be treated with anti-protozoan drugs. In NW most people seem to contract it from drinking from "pure mountain streams". With the increased human population, it is unwise to consider that any drinking water in the "wild" is safe any longer. Humans generally do not bury their fecal wastes in the woods deep enough to prevent them being washed into nearby streams and lakes. In addition the animals reservoirs continually contaminate the streams and lakes. 


AMEBIASIS

The protozoan disease caused by Entamoeba histolytica is mainly a disease in the US of poverty and sexual promiscuity. This disease is mainly found among male homosexuals, in the poor South, on some Indian reservations and among migrant farm workers. It is endemic worldwide and is mostly found in crowded, unsanitary conditions. The etiological agent is an amoeba that is capable of forming a CHITIN COVERED CYST to resist adverse environmental conditions.

The disease, AMOEBIC DYSENTERY, is characterized by diarrhea, abdominal pain and blood in the feces, however it is rarely fatal. Rather, it is CHRONIC INFECTION that ultimately shortens lives. It is primarily transmitted by fecally polluted water. The organism can invade other tissues including the liver resulting in severe liver damage and early death. It can be treated by anti-protozoan drugs, but once it leaves the intestine it is often impossible to cure. 



 

SUMMARY OF WBD

FAQ: "If it is so easy to prevent WBDs, why are they still such a worldwide problem?"

 


ANSWER: The cheap answer is because so many humans remain ignorant of basic sanitation procedures and/or are too stubborn to apply them. Certainly, ignorance is a major cause of WBDs. However, even people who know the proper sanitation measures are often unable to apply them because of a lack of personal or community resources. Good sanitation is equally a personal and community thing. It is of little help if you are personally clean, but you are immersed in a world of contaminated food and water. No matter how vigilant you are it is likely that you will contact a WBD. Often governments feel it is better to spend their limited resources on arms or industrial development rather than on a clean water or food supply. Where the mass of the population is poor, the wealthy often refuse to help pay for sanitation. As you will see in one of the tapes (Cell Wars) shown in lab, sanitary measures are difficult to apply. 



 


Did you hear about the doctor who divorced his wife when he learned that she was eating an apple-a-day?


BIORÉHABILITATION

La bioréhabilitation (biorémédiation) est définie comme étant l'utilisation de micro-organismes pour le traitement de la pollution environnementale.

Le principe de base de la bioréhabilitation est le même que pour le traitement des effluents: utiliser le métabolisme des micro-organismes pour métaboliser des polluants et les convertir en composés innofensifs. La bioréhabilitation passe généralement par les étapes suivantes:

Définir le problème de pollution: quelles sont les substances présentes; en quelles quantités; quelle est leur toxicité; s'accumulent-elles et si oui, à quel rythme?

Développer une approche de traitement basée sur les micro-organismes.

Isoler un(des) micro-organismes qui métabolisera(ont) le(s) polluants(s).

Faire croître une population importante de ces micro-organismes.

Introduire ces micro-organismes dans l'environnement pollué en leur donnant les éléments nutritifs et les conditions de croissance optimales.

L'élément essentiel consiste à trouver les BONS MICROBES. Un moyen d'y arriver consiste à employer une TECHNIQUE D'ENRICHISSEMENT DE CULTURE:

  1. Fabriquer un milieu de culture où le polluant à traiter constitue la source de carbone majeure (ou unique)
  2. Introduire dans ce milieu un inoculum naturel, riche en biomasse diversifiée(Ex: un échantillon de sol de jardin, ou prélevé sur le site à décontaminer)
  3. Incuber et suivre la concentration en polluant. Si le milieu contient un microbe qui peut métaboliser le polluant, ce micro-organisme devrait se reproduire plus rapidement que les autres (par sélection naturelle).
  4. Inoculer une nouvelle culture à partir de l'ancienne, possiblement en augmentant la concentration relative du polluant. Répéter le processus jusqu'à ce que le micro-organisme dépolluant prédomine.
  5. Isoler et étudier le micro-organisme afin d'optimiser ces conditions de croissance.
  6. Utiliser ce micro-organisme tel que décrit ci-haut.
La bioréhabilitation s'est avérée efficace pour le traitement de problèmes de pollution tels que les sols contaminés au carburant, huile et créosote, certains préservatifs du bois, pour le traitement des marées noires, etc. C'est un procédé couteux, mais qui, dans plusieurs cas, est plus efficace que d'autres techniques telles que l'enfouissement, le traitement chimique ou thermique.

Copyright © Dr. R. E. Hurlbert, 1996. This material may be used for educational purposes only and may not be duplicated for commercial purposes.

HURLBERT:

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PHONE: 509-335-5108
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E-mail address: hurlbert@wsu.edu hurlbert@pullman.com
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